Stanford University Hiring for Life Science Technician 1 at Stanford, California Full Time

Life Science Technician 1

School of Humanities and Sciences, Stanford, California, United States

New

Research

Post Date 23 hours ago

Requisition # 91481

This position is represented by SEIU Local 2007 and the collective bargaining agreement between the university and SEIU Local 2007 governs the terms and conditions of employment.

Life Science Technician 2

The Dixon lab (http://www.dixonlaboratory.com) has a position for a motivated Life Science Technician 2 level with interests in cancer, cell biology, or chemical biology. Our lab focuses on identifying and characterizing new cell death pathways (e.g. ferroptosis) that can potentially be activated to treat cancer. A major goal of the lab is to understand how new small molecules we have discovered in the lab can induce ferroptosis in cancer cells and seeking to understand whether this involves known or novel molecular mechanisms. Research in the lab is driven by in vitro approaches with a particular focus on the effects of different small molecules on cancer cell death. This position will provide opportunities to learn a range of cell biology and chemical biology techniques, and offers a unique opportunity to collaborate with other scientists interested in genomics, biochemistry and computational biology.

The main responsibilities of the successful candidate will be culturing cancer cells of different genotypes and examining the effects of lethal compound treatment. In addition, follow-up studies examining the effects of deleting specific genes using CRISPR/Cas9 technology on drug-induced cell death will be pursued. Other responsibilities will include performing routine laboratory techniques such as DNA cloning, as well as maintaining excellent records of lab work, curating samples, and assisting in data analysis. This is a two-year fixed-term appointment with the possibility to renew.

Characteristic Duties:

The duties listed under this section are designed to provide a representative sampling of key tasks and/or responsibilities associated with the job. They are not intended to be a complete list of all the duties performed by employees in the classification.

Perform animal surgical techniques such as suturing, catheterization and infusion of arteries, small organ dissection, organ perfusion and tumor implantation and passage in small animals.

• Perform animal surgical techniques such as suturing, catheterization and infusion of arteries, small organ dissection, organ perfusion and tumor implantation and passage in small animals.

• Immunohistological procedures including frozen sectioning and multi-step immunologic staining for fluorescence or enzymatic detection. Some level of histologic assessment may be required.

• Maintenance, transfer and establishment of cell lines from normal or tumor cells, preparation and bioassay of serum, screening for contamination, isolation of cell mutants, cell staining, mass production of cells and cell cloning.

• Fixation, dehydration, staining and embedding of specimens for electron microscopy; carbon coating, negative staining, shadowing, operation of electron microscope (EM) either transmission or scanning; use of an ultramicrotome with a diamond knife for routine thin sectioning; alignment of an EM where the system is automated.

• Purification and /or structural characterization of compounds for which a protocolis well established.

• Separation of cells, organelles, or molecules using density gradients.

• Radioimmunoassay of viruses, proteins, or hormone levels in cell extract according to established protocol.

• Techniques such as electrophoresis, chromatography, autoradiography, radiolabeling of animals, cell cultures, or macromolecules.

• Isolation, purification and maintenance of mammalian viruses and bacteriophages; determination of viral concentration.

• Genetic manipulation of bacteria including mutagenesis, ‘phage transduction and transformation.

• Accurate documentation of experimental protocols and basic statistical analysis of data.

Minimum Education and Experience Required

Two years of laboratory experience or two years of college coursework in the scientific discipline a combination of equivalent education and relevant work experience.

Minimum Qualifications

Work at the Life Science Technician II level requires experience and ability beyond that required for the Life Science Technician I, including increased technical skills and problem-solving abilities.

Physical Requirements

• Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand.

• Ability to stand and move on hard surfaces for up to eight hours.·Occasionally lift and handle materials up to 10 pounds

• Rarely carry/pull and/or push up to 20 pounds·Must have correctible vision to perform duties of the job.

• Position may require repetitive motion.

Working Conditions

Work occurs in a research laboratory environment. Depending on research, may work in the vicinity of animals, chemicals, gasses, or hazardous materials. Expected to follow all safety requirements and procedures including wearing appropriate Personal Protective Equipment (PPE) if required.

Work Standards

• Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.

• Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors based on training and lessons learned.

• Subject to and expected to comply with all applicable University policies and procedures, including but not limited to thepersonnel policies and other policies found in the University’s Administrative Guide, http://adminguide.stanford.edu/.and the appropriate collective bargaining agreement at https://cardinalatwork.stanford.edu/hr-processes-policiespolicies/labor-relations-collective-bargaining.

Additional Information

• Schedule: Full-time

• Job Code: 4894

• Employee Status: Fixed-Term

• Grade: K

• Requisition ID: 91481