University of Rochester Hiring for Laboratory Technician IV (S) – 240222 at Rochester Full Time

Laboratory Technician IV (S) Job ID 240222

Location

School of Medicine & Dentistry

Full/Part Time Full-Time

Favorite Job

Regular/Temporary Regular

Opening

Full Time 40 hours Grade 079 Surgery Research

Responsibilities

GENERAL PURPOSE:

Will provide technical support for experiments performed in a Tumor Immunology Laboratory. Projects in the lab focus on understanding predictive biomarkers and mechanisms of cancer-related muscle wasting. Major histology studied include muscle, diaphragm, intestine, and pancreatic cancer. The PI is a muscle physiologist in the division of surgical oncology. The Technician should be expected to work independently, organize and troubleshoot experiments, and integrate different experimental approaches to address scientific aims. The Technician must be able to work in a collaborative lab environment internal and external to the Division and Department.

The Technician will plan and perform routine molecular and immunobiology techniques as well as technical functions including the development and maintenance of laboratory safety protocols, experimental protocols, purchase and maintenance of laboratory supplies, equipment and budgeting. The Technician will oversee and train new laboratory personnel including undergraduate and graduate students in routine research techniques. Working with human cell culture and mouse models will be required. Results from experiments performed by the Technician will be used both in publications and grant proposals.

MAJOR DUTIES AND RESPONSIBILITIES:

Immunobiology Techniques

Isolate cells from primary mouse muscle and analyze cells by flow cytometry, CyTOF and immuno-cytochemistry, analyze mouse and human serum and using ELISA and Luminex multiplex immunoassays.

Molecular Biology Techniques

Develop, implement, and troubleshoot experiments utilizing molecular biology techniques, including isolation of RNA and DNA, PCR, RT_PCR, and qPCR.

Cell Biology Techniques

Culture, treat, and transfect (CRISPR, shRNA) immortalized cell lines using basic cell culture techniques.

Analyze data, provide written summaries of data for grants and publications; develop figures for grants and publications. Use software applications to generate test reports; present results including results of literature reviews to supervisor and PI and at lab meetings with the research team. Prepare figures and tables for grant applications or to present data. Critically appraises research methodology and identifies issues or potential for issues and makes recommendations to PI and Supervisor for resolutions. Prepares basic statistical testing including graph generation. Assists with budget preparation.

Prepare general buffers and media. Oversee maintenance, management, and/or repair of equipment. Independent management of the laboratory’s chemical inventory. Monitor and coordinates ordering of lab inventories ensuring orders for equipment and supplies as properly processed. Train individuals in the appropriate use of equipment. Has the ability to make decisions on purchases of supplies and all equipment. Will perform in depth comparative research to obtain the best product at the best price and provide informed recommendations to the PI and Supervisor for decision making. Meets with vendors to review equipment/laboratory needs. Responsible for orderly appearance of the laboratory.

Train, supervise new lab personnel and students; direct and assist laboratory personnel, undergraduate and graduate students and technicians in the laboratory in terms of execution and troubleshooting of their experimental work including research compliance around animal care and routine research techniques. Monitor quality of day-to-day work of these individuals. With supervisor, involved in interviewing and hiring decisions; evaluating performance and resolving performance issues.

• Provide instruction to other laboratory personnel in BSL2 procedures and general laboratory research techniques. Orients new and collaborating laboratory personnel to laboratory-related procedures.

  Attends weekly laboratory meetings and relevant seminars pertinent to the research goals of the laboratory. Meets with PI on a regular basis to discuss research findings and make recommendations regarding future directions.

  Other duties as assigned.

  QUALIFICATIONS:

Associates degree required

2-3 years specialized experience in related field or equivalent combination of education and experience required

Preferred:

• Advanced computer skills including MS Word and Excel, required. Experience using flow cytometry software, statistical software packages such as Prism are preferred.

• Knowledge of general laboratory safety processes, universal precautions, aseptic cell culture technique, handling of mice and practice of rodent euthanasia required. Thorough documentation of results in written and electronic database formats will be required. Skills in basic database management and generation of publication-quality figures preferred. Needs to be proficient in anesthetic techniques.

The University of Rochester is committed to fostering, cultivating, and preserving a culture of equity, diversity, and inclusion to advance the University’s mission to Learn, Discover, Heal, Create – and Make the World Ever Better. In support of our values and those of our society, the University is committed to not discriminating on the basis of age, color, disability, ethnicity, gender identity or expression, genetic information, marital status, military/veteran status, national origin, race, religion/creed, sex, sexual orientation, citizenship status, or any other status protected by law. This commitment extends to the administration of our policies, admissions, employment, access, and recruitment of candidates from underrepresented populations, veterans, and persons with disabilities consistent with these values and government contractor Affirmative Action obligations.

How To Apply

All applicants must apply online.

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